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NASC

The European Arabidopsis Stock Centre

NASC Stock Detail Page

NASC ID: N9902

Name: upf1-5

ABRC stock number: CS9902

Description: Homozygous line derived from SALK_112922. TDNA insertion located in the 3 prime UTR of UPF1 at position 7037. UPF1 mRNA has been severely reduced (RT-PCR).

Donation Date: 2010-02-03

Donated by:  University of Leeds Brendan Davies

Donor Number: upf1-5

Stock type: individual line

Material type: seed

Status Price (£)
Available £11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-0(N60000, SALK_112922)

Segregation status: homozygous

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:
  • upf1-5
  • upf1

Phenotype

Over-representation of PTC-containing mRNAs. Flowering time affected approximately 2 weeks later than wild-type plants in long-day growth conditions. upf1-5 also has narrower and more jagged rosette leaves than wild-type plants. In approximately 5% of the flowers, upf1-5 exhibits aberrant flower formation. Two or three flowers appear to be initiated at the same point, giving rise to the appearance of double and triple fused flowers. Sometimes new flowers are initiated within developing flowers, resulting in the formation of a series of distorted flowers at different stages of development. (Arciga-Reyes et al., 2006). Phenotype can vary with conditions

References

Arciga-Reyes, L. et al. 2006. UPF1 is required for nonsense-mediated mRNA decay (NMD) and RNAi in Arabidopsis. The Plant Journal 47(3):480-489.PubMed ID: 16813578.